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Anti-Glial Derived Neurotrophic Factor Antibody

品牌
Sigma-Aldrich
货号
AB5252
规格纯度
serum, Chemicon®
参考价格
6022.61 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Specific for GDNF. No cross reactivity with Neurturin.

免疫原

Recombinant human GDNF.

应用

Research Category
Neuroscience
Anti-Glial Derived Neurotrophic Factor Antibody detects level of Glial Derived Neurotrophic Factor & has been published & validated for use in WB.
Immunoblotting: 1:3,000 using TBS (not PBS) and blocking is best performed with IgG free BSA highest quality. Antibody recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32 kDa (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCL until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.

Optimal working dilutions must be determined by end user.
Research Sub Category
Neurochemistry & Neurotrophins

Neuroinflammation & Pain

外形

Sheep serum. Lyophilized. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue. Contains no preservative. Glycerol (1:1) can be added for additional stability.

储存及稳定性

Maintain lyophilized material at -20°C to -70°C (dry) for up to twelve months. After reconstitution maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源sheep
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityrat, human, mouse
manufacturer/tradenameChemicon®
technique(s)western blot: suitable
NCBI登记号NM_000514.2
NM_199231.1
NM_199234.1
UniProt登记号P39905
运输dry ice
Gene Informationhuman ... GDNF(2668)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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