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Anti-CD133 Antibody, clone 13A4

品牌
Sigma-Aldrich
货号
MAB4310
规格纯度
clone 13A4, Chemicon®, from rat
参考价格
6190.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Prominin is a novel plasma membrane protein with an N-terminal extracellular domain, five transmembrane segments flanking two short cytoplasmic loops and two large glycosylated extracellular domains, and a cytoplasmic C-terminal domain. DNA sequences from Caenorhabditis elegans predict the existence of a protein with the same features, suggesting that prominin is conserved between vertebrates and invertebrates. Prominin is found not only in the neuroepithelium but also in various other epithelia of the mouse embryo. In the adult mouse, prominin has been detected in the brain ependymal layer, and in kidney tubules. In these epithelia, prominin is specific to the apical surface, where it is selectively associated with microvilli and microvilli-related structures. Remarkably, upon expression in CHO cells, prominin is preferentially localized to plasma membrane protrusions such as filopodia, lamellipodia, and microspikes. These observations imply that prominin contains information to be targeted to, and/or retained in, plasma membrane protrusions rather than the planar cell surface. Moreover, results show that the mechanisms underlying targeting of membrane proteins to microvilli of epithelial cells and to plasma membrane protrusions of non-epithelial cells are highly related. {Weigmann, A et al.1997}. Also see {http://www.ncbi.nlm.nih.gov/prow/guide/438375806_g.htm} for more information on CD133 and prominin expression.

特异性

Recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural & endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown.

This antibody can be used to isolate murine stem cells from brain and bone marrow.

The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen. The original paper is located at: http://www.pnas.org/cgi/reprint/94/23/12425.pdf

免疫原

Telencephali of ten 12-day-old NMRI mouse embryos (E12, ~1 mg protein), which consist mostly of neuroepithelium, were homogenized in 1 ml PBS, mixed with crushed nitrocellulose filter (1 cm2 in 0.5 ml PBS) as adjuvant, and injected i.p. into a LouXSD rat. {Weigmann et al, PNAS 1997}

应用

Research Sub Category
Neural Stem Cells

Hematopoietic Stem Cells
Anti-CD133 Antibody, clone 13A4 detects level of CD133 & has been published & validated for use in FC, IH, IP & WB.
Research Category
Stem Cell Research

目标描述

115 kDa

外形

Format: Purified
Protein A purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

储存及稳定性

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Positive Control: Kidney tissue or membranes
Negative Control: Liver tissue or membranes

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆13A4, monoclonal
species reactivitymouse
should not react with rat, chicken, Drosophila
manufacturer/tradenameChemicon®
technique(s)flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
inputsample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
同位素/亚型IgG1κ
适用性not suitable for immunohistochemistry (Paraffin)
NCBI登记号NM_006017.1
UniProt登记号O43490
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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