产品说明
一般描述
Nck-2 is an adaptor protein that regulates signal transduction in cells. This protein consists of one C-terminal SH2 domain and three N-terminal SH3 domains. Nck-2 interacts with several growth factor signaling components such as EGFR, IRS-1 and PDGFR, and also with the integrin-linked kinase signaling protein, LINCH .
Monoclonal Anti-Nck-2 (mouse IgG2b isotype) is derived from the 8.8 hybridoma produced by the fusion of mice myeloma cells and splenocytes from BALB/c mice immunized with a C-terminal region of human Nck-2 protein fused to glutathione S-transferase (GST). NCK adaptor protein 2 (Nck-2) is a 43 kDa protein, that is 68 % identical at the protein level as that of Nck-1.
特异性
The epitope recognized by the antibody resides within amino acids 176-274 of human Nck-2. Cross reactivity has been observed with human Nck-1. Monoclonal Anti-Nck-2 is specific for human, rabbit, rat and chicken Nck-2 (approx. 43 kDa). The product also binds to human Nck-1.
免疫原
C-terminal region of human Nck-2 protein (amino acids 115-380) fused to GST
应用
Monoclonal Anti-Nck-2 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
生化/生理作用
NCK adaptor protein 2 (Nck-2) can interact with kinases like the focal adhesion kinase (FAK), which is a cytoplasmic tyrosine kinase involved in the cellular control of motility. It stimulates human melanoma cell proliferation.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02097007 |
| NACRES | NA.44 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 8.8, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 43 kDa |
| species reactivity | human, rabbit, rat, chicken |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/mL using rat tongue extract |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | O43639 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... NCK2(8440) rat ... Nck2(316369) |
