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Anti-Apaf-1 Antibody, clone 13F11

品牌
Sigma-Aldrich
货号
MAB3504-I
规格纯度
clone 13F11, from rat
参考价格
3507.4 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.

特异性

Clone 13F11 specifically detects Apaf-1 in mouse and rat. It targets an epitope within the first 97 amino acids in the N-terminal region.

免疫原

A recombinant fragment corresponding to the first 97 amino acids of mouse Apaf-1.

Epitope: N-terminus

应用

Western Blotting Analysis: 1 ug/mL from a representative lot detected Apaf-1 in mouse bone marrow tissue lysate.

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Vaughn, A.E., et. al. (2007). Cell Death Differ. 14(5):973-81; Wright, K.M., et. al. (2004). J Cell Biol. 167(2):303-13).

Research Category
Apoptosis & Cancer
Anti-Apaf-1, clone 13F11, is a rat monoclonal antibody that detects Apaf-1 in mouse and rat. It has been tested for use in Western Blotting.

质量

Evaluated by Western Blotting in mouse lymph node tissue lysate.

Western Blotting Analysis: 1 ug/mL of this antibody detected Apaf-1 in mouse lymph node tissue lysate.

目标描述

~141 kDa observed; 141.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: MAB3504

外形

Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆13F11, monoclonal
species reactivityrat, mouse
包装antibody small pack of 25 μg
technique(s)western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_001036023
UniProt登记号O88879
运输ambient

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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