产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
免疫原
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human dimethyl Histone H3 methylated at Lys36.
应用
Research Category
Epigenetics & Nuclear Function
Anti-dimethyl Histone H3 Antibody (Lys36) is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting, Dot Blot & Chromatin IP (ChIP).
Dot Blot Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H3 (Lys36), but not unmethylated Histone H3 or other peptides corresponding to modified histones.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot imunoprecipitated dimethyl Histone H3 (Lys36) in HeLa cell lysate.
质量
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected dimethyl Histone H3 (Lys36) in 10 µg HeLa acid extract and demonstrated a loss of signal in 0.5 µg of unmethylated recombinant Histone H3.
目标描述
~17 kDa observed
外形
Unpurified
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | serum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | vertebrates (based on 100% sequence homology) |
| technique(s) | ChIP: suitable dot blot: suitable western blot: suitable |
| NCBI登记号 | NP_003484 |
| UniProt登记号 | Q16695 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
