Anti-CD62E [ELAM-1] Antibody, clone 1.2B6

产品说明

一般描述

Selectins (CD62s) are single-chain transmembrane glycoproteins that function as cell adhesion molecules (CAMs) by interacting with a range of oligosaccharide structures including sialyl-Lewis X (sLeX) via their C-type lectin domain. All three known selectins (L-, E-, and P-selectin or CD62L CD62E, CD62P, respectively) share a similar overall domain structure that includes an N-terminal C-type lectin domain (calcium-dependent lectin domain), an EGF-like domain, a variable number of short consensus repeat (SCR) or Sushi domains (2, 6, and 9 for CD62L CD62E, CD62P, respectively), a transmembrane domain, and a cytoplasmic tail. The extracellular domains show a high homology among the three selectins, the transmembrane and cytoplasmic domains however are not conserved among the three. E-selectin (CD62E, ELAM-1) and P-selectin (CD62P, GMP-140, LECAM3, PADGEM) are mainly expressed on endothelial cells at inflammatory sites, whereas L-selectin (CD62L) is exclusively expressed on leukocytes.

特异性

This antibody is specific to human antigen E-selectin (ELAM-1, 115 kDa) and P-selectin that are expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. This antibody inhibits neutrophil adhesion to E-selectin and P-selectin when used as F(ab′)2 fragments. Although previously thought to recognize only human CD62E, later data (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560) showed that this antibody also recognizes human CD62P by binding to a common epitope on these members of the selectin family.

应用

Anti-CD62E [ELAM-1] Antibody, clone 1.2B6 is an antibody against CD62E [ELAM-1] for use in Immunohistochemistry, ELISA, Flow Cytometry, Western Blotting, Activity Assay.
ELISA Analysis: A representative lot detected an induction of E-selectin expression on HUVECs by T-cells isolated from human PBMCs (Yarwood, H., et al. (2000). J. Leukoc. Biol. 68(2):233-242).
ELISA Analysis: A representative lot detected a time-dependent induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs, but not A549 cells (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Flow Cytometry Analysis: A representative lot specifically reacted with exogenously expressed human E- and P-, but not L-, selectin on the surface of transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Flow Cytometry Analysis: A representative lot detected an induction of E-selectin expression by IL-1 or TNF stimulation on HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).
Western Blotting Analysis: A representative lot specifically reacted with E- and P-, but not L-, selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked the adhesion of HL-60 cells to E- or P-selectin transfected cells (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Activity Assay: A representative lot blocked sialyl-Lewis X (sLeX) binding by E- and P-selectin IgG fusion proteins (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560).
Immunohistochemistry Analysis: A representative lot detected ELAM-1 immunoreactivity in acetone-fixed frozen human skin tissue sections (Groves, R.W., et al. (1991). Br. J. Dermatol. 124(2):117-123).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E-selectin and associated proteins from lysates of TNF-stimulated HUVECs (Wellicome, S.M., et al. (1990). J.Immunol. 144(7):2558-2565).

质量

Evaluated by Immunohistochemistry in human tonsil tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CD62E [ELAM-1] in human tonsil tissue.

目标描述

66 kDa calculated.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息