产品说明
应用
The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.
Enzyme used in determining cholesterol in serum
包装
500 units in poly bottle
生化/生理作用
Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.
Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.
单位定义
One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 ℃.
外形
Supplied as a lyophilized powder
基本信息
| 酶学委员会(EC)编号 | 1.1.1.B40 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in Streptomyces lividans |
| 形式 | lyophilized solid |
| specific activity | ≥18 unit/mg solid |
| 分子量 | ~37 kDa by SDS-PAGE |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H334 |
| 预防措施声明 | P261 - P284 - P304 + P340 + P312 - P501 |
| 危险分类 | Resp. Sens. 1 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
