产品说明
一般描述
Anti-Ciliated Cell Marker antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma LhS 28 produced by the fusion of mouse myeloma cells (Sp2/0 cells) and splenocytes from BALB/c mice.
特异性
Anti-Ciliated Cell Marker antibody, Mouse monoclonal recognizes an antigen in human and hamster ciliated cells.
免疫原
BHK a21 cytoskeletal preparation.
应用
May be used in the detection of ciliated cells and for investigating mechanisms of ciliogenesis.
Anti-Ciliated Cell Marker antibody, Mouse monoclonal may be used in:
- immunoelectron microscopy
- immunoblotting
- immunohistochemistry
- immunocytochemistry
生化/生理作用
The mouse hybridoma LhS 28 secretes a monoclonal antibody that recognizes the antigen 44-45 kDa protein associated with the ciliary basal bodies. The antigen recognized by LhS 28 is present in the 9+0 microtubule arrays in the basal body structure of the cilium. It serves as an unequivocal marker for in situ detection and in vitro studies of human ciliated epithelial cells. LhS28 is a potential marker for tubal metaplasia identification and may be useful in recognizing low grade cervical glandular intraepithelial neoplasia. It is effective in detecting ciliogenesis and ciliated cells associated with normal and pathological human specimens
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD08705577 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | LhS 28, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~45 kDa |
| species reactivity | human, hamster |
| 浓度 | ~2 mg/mL |
| technique(s) | electron microscopy: suitable immunocytochemistry: 1-2 μg/mL using BHK a21 cells immunohistochemistry: suitable microarray: suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
