产品说明
一般描述
Anti-Calnexin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TO-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Calnexin contains a long (461 amino acids) N-terminal Ca2+-binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) trans-membrane segment and an acidic cytosolic domain (96 amino acids).
Calnexin (p88, IP90) is a calcium-binding, type I integral membrane protein, localized primarily in the endoplasmic reticulum (ER). Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. The lectin specificity of calnexin and its soluble homologue calreticulin has been identified as high mannose oligosaccharides terminating in monoglucosyl residues linked through α1-3. Calnexin has been shown to be associated with several cell surface proteins, including MHC class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the ER. It also forms complexes with other resident ER proteins involved in Ca2+-dependent retention of proteins.
特异性
Mouse monoclonal clone TO-5 anti-Calnexin antibody recognizes human Calnexin, ~90 kDa.
应用
Anti-Calnexin antibody, Mouse monoclonal has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- flow cytometry
- immunocytochemistry
- immunohistochemistry
- immunofluorescence analysis
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01094399 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | TO-5, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~90 kDa |
| species reactivity | human |
| 浓度 | ~2 mg/mL |
| technique(s) | flow cytometry immunocytochemistry immunohistochemistry indirect ELISA western blot |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P27824 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... CANX(821) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
