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Protein Kinase A catalytic subunit human

品牌
Sigma-Aldrich
货号
C8482
规格纯度
>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
参考价格
11383.1 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

生化/生理作用

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

外形

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

分析说明

One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 ℃ (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 ℃, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 ℃, pH 7.4.

基本信息

MDL编号MFCD03458215
NACRESNA.32

产品性质

质量水平200
重组expressed in E. coli
测定>90% (SDS-PAGE)
形式buffered aqueous glycerol solution
specific activity>1000 units/mg protein
分子量 43.5 kDa
UniProt登记号P17612
运输dry ice
储存温度−70℃
Gene Informationhuman ... PRKACA(5566)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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