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Cyclohexanone Monooxygenase from Acinetobacter sp.

品牌
Sigma-Aldrich
CAS
52037-90-8
货号
C1622
规格纯度
≥12 U/mL, ammonium sulfate suspension, recombinant, expressed in E. coli
参考价格
1824.58 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

Cyclohexanone monooxygenase has been used in a study that cloned and overexpressed the 2-oxo-Δ(3)-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) in Escherichia coli. Cyclohexanone monooxygenase has also been used in a study that investigated the effects of structural modification of the cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells.

生化/生理作用

Purified cyclohexanone monooxygenase has a molecular weight of 59,000 Da. It is a versatile oxygenation catalyst. The enzyme uses the bound FAD-4a-OOH oxygenating intermediate to initiate transfer of oxygen to electrophilic substrate sites. The reaction consequently yields the corresponding sulfoxide and selenoxide products. This enzyme is also capable of oxygenating at nitrogen, trivalent phosphorus, and boron sites in boronic acids. Hence, it is one of the most broad-based flavoprotein oxygenases known.

单位定义

1 unit corresponds to the amount of enzyme which catalyzes the cyclohexanone-stimulated oxidation of 1 μmol of NADPH per minute at pH 9.0 and 30 ℃

外形

Suspension in 80% saturated ammonium sulfate, 20 mM K-Na-phosphate buffer pH 7, 3.5 mM 1,4-Dithioerythritol (DTE)

基本信息

酶学委员会(EC)编号1.14.13.22 ( BRENDA | IUBMB )
MDL编号MFCD00146601
NACRESNA.54

产品性质

质量水平200
重组expressed in E. coli
形式ammonium sulfate suspension
浓度≥12 U/mL
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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