产品说明
应用
Creatinine Deiminase microbial has been used:
- to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
- in creating creatinine-sensing membrane for biophysical studies
- to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
包装
250 units in poly bottle
生化/生理作用
Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.
物理属性
Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75℃
pH Stability : pH 7.0 – 11.0 (30℃, 20hr)
Thermal stability : Below 65℃ (pH 7.5, 1hr)
单位定义
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 ℃ in a coupled system with
外形
Lyophilized powder containing mannitol as stabilizer
基本信息
| 酶学委员会(EC)编号 | 3.5.4.21 ( BRENDA | IUBMB ) |
| EC 号 | 253-449-1 |
| MDL编号 | MFCD00130878 |
| NACRES | NA.54 |
产品性质
| 形式 | lyophilized powder |
| 质量水平 | 200 |
| specific activity | ≥25 units/mg protein |
| 分子量 | ~260 kDa |
| 组成 | Protein, ≥15% biuret |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
