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Anti-Aly antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
A9979
规格纯度
clone 11G5, purified from hybridoma cell culture
参考价格
4731.57 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Anti-Aly antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 11G5 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from BALB/c mice immunized with recombinant human Aly/REF protein. Aly is also known as mREF1-I which is an RNA-binding protein that contains an RNA-binding domain and is recruited to the mRNA-protein complex (mRNP) complexes during the mRNA splicing.
Aly is a nuclear chaperone protein that may modulate DNA binding, mRNA synthesis, and dimerization of basic region-leucine zipper proteins. Mouse Monoclonal Anti-Aly antibody recognizes human and Xenopus Aly.

免疫原

recombinant human Aly/REF protein.

应用

Mouse Monoclonal Anti-Aly antibody can be used for immunocytochemistry, immunoprecipitation, indirect ELISA, microarray and western blot (0.5-1.0μg/ml) assays.
Co-immunoprecipitation and western blot studies were performed in Hela cells using a monoclonal mouse anti-Aly antibody. The results indicated that Aly which was observed as a 30 kDa band interacted with the Herpes Simplex Virus I protein, ICP27.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

生化/生理作用

Excess of recombinant expression of Aly in the cells increases both the rate and efficiency of spliced and unspliced mRNA export (in vivo) from the nucleus to the cytoplasm. Aly is dissociated from the mRNAs after the export to the cytoplasm.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD07785404

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆11G5, monoclonal
形式buffered aqueous solution
分子量antigen ~30 kDa
species reactivityhuman, Xenopus
浓度~2 mg/mL
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of HeLa cells
同位素/亚型IgG1
运输dry ice
储存温度−20℃
Gene Informationhuman ... THOC4(10189)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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