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Anti-VEGF Antibody

品牌
Sigma-Aldrich
货号
07-1420
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5352.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

特异性

Recognizes Vascular Endothelial Growth Factor (VEGF), a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability.

免疫原

Highly pure (>98%) recombinant mouse Vascular Endothelial Growth Factor.

应用

Immunohistochemisty (paraffin): VEGF staining in mouse placenta, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mouse VEGF (50.0 ng/mL), a concentration of 1.0-2.0 µg/mL of a previous lot of this antibody was required.

ELISA: A concentration of at least 0.5 µg/mL (100 μL/well) is required to detect 0.2-0.4 ng/well of recombinant mouse VEGF using a previous lot of this antibody.

Optimal working dilutions must be determined by the end user.

Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
Anti-VEGF Antibody detects level of VEGF & has been published & validated for use in WB, IH(P), ELISA.

质量

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis:
1:500 dilution of this lot detected VEGF on 10 μg of Huvec lysates.

目标描述

27 kDa

联系

Replaces: AB2142P

外形

Affinity purified
Affinity purified rabbit polyclonal IgG employing immobilized mouse VEGF matrix. Antibody solution (1 mg/ml) was lyophilized from PBS, pH 7.2. Reconstitute with sterile distilled water.

储存及稳定性

Reconstituted antibody, at a concentration of ≥ 0.2 mg/mL, is stable for at least six weeks at 2-8ºC. Aliquots stored at -20ºC are stable for at least one year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Huvec cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman, mouse
technique(s)ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG
NCBI登记号NM_001025366.1
UniProt登记号P15692
运输dry ice
Gene Informationhuman ... VEGFA(7422)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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