产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
Recognizes monomethyl-histone H3 (Lys4), Mr 17kDa.
Broad species to cross-reactivity is expected.
免疫原
Epitope: Lys4
KLH-conjugated, synthetic peptide containing the sequence ART[meK]…, in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3.
应用
Anti-monomethyl-Histone H3 (Lys4) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H3 (Lys4) also known as H3K4me1, Histone H3 (mono methyl K4) & has been validated in ICC, IF, PIA, WB, ChIP-seq, DB.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry:
Reported by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
质量
Routinely evaluated by Western Blot in acid extracted proteins from HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
目标描述
17 kDa
外形
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
Format: Purified
Protein A purified
储存及稳定性
Stable for 1 year at 2 to 8°C from date of receipt.
分析说明
Control
HeLa cell extract
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | human, vertebrates |
| manufacturer/tradename | Upstate® |
| technique(s) | ChIP: suitable (ChIP-seq) dot blot: suitable immunocytochemistry: suitable immunofluorescence: suitable inhibition assay: suitable (peptide) western blot: suitable |
| 同位素/亚型 | IgG |
| NCBI登记号 | NM_003493 |
| UniProt登记号 | Q16695 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
