Anti-Nedd4

产品说明

一般描述

E3 ubiquitin-protein ligase NEDD4 (UniProt: P46934; also known as EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase Nedd4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4) is encoded by the Nedd4 (also known as KIAA0093, Nedd4-1) gene (Gene ID: 4734) in human. NEDD4 is a peripheral membrane protein that is recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, it follows IGF1R internalization and remains associated with early endosomes. Nedd4 can accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. It is shown to specifically ubiquitinate Lysine 63 in target proteins. It also monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. NEDD4 is reported to polyubiquitinate the epithelial sodium channel and any mutations that disrupt association between Nedd4 and sodium channel can lead to Liddle s syndrome, a hyperaldosteronism-like hypertension disease. NEDD4 can interact with viral proteins that contain a late-budding motif P-P-P-Y and this interaction is believed to be essential for viral particle budding of many retroviruses, like HTLV-1 Gag, and MLV Gag. It contains four WW domains (WW 1-4; aa 610-643; 767-800; 840-873; and 892-925) and one HECT domain (aa 984-1318). The WW domains mediate its interaction with PPxY motif-containing proteins.

特异性

This rabbit polyclonal antibody specifically detects E3 ubiquitin-protein ligase Nedd4.

免疫原

GST/His-tagged recombinant fragment corresponding to 68 amino acids from the N-terminal half of human Nedd4.

应用

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected NEDD44 in rat cerebral cortex, rat cerebellum, and human colon cancer tissue sections.

Anti-Nedd4 Antibody, Cat. No. 07-049, is a rabbit polyclonal antibody that detects Nedd4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

质量

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nedd4 in rat kidney tissue lysate.

目标描述

~110 kDa observed; 102.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Format: Purified
Protein A purified
Purified rabbit polyclonal antibody in buffer containing 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 0.05% sodium azide.

分析说明

Control
Kidney cytoplasmic extracts.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息