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Anti-JMJD3 (NT) Antibody

品牌
Sigma-Aldrich
货号
07-1533
包装型号
规格纯度
from rabbit
参考价格
6954.73 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

JMJD3 (Jumonji domain-containing protein 3) is part of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. JMJD3 specifically demethylates Lys27 on histone H3, demethylating both the trimethylated and dimethylated form. JMJD3 plays a role in the regulation of posterior development by regulating HOX gene expression. It is also involved in the inflammatory response by regulating gene expression and macrophage differentiation

特异性

Not tested in other species.
Recognizes the N-terminal region of human JMJD3. Additional lower MW bands are detected in some lysates that may be degradation products.

免疫原

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3.
Epitope: N-terminus

应用

ELISA:
This antibody is reported by an
outside laboratory to be suitable for ELISA.

Immunohistochemistry:
This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry.

Immunocytochemistry Analysis:
Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green).

Western Blot Analysis:
The antibody recognizes JMJD3 in HeLa cell lysates.

Use Anti-JMJD3 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ICC, ELISA, WB to detect JMJD3 (N-terminus) also known as JmjC domain-containing protein 3.
Research Sub Category
Histone Modifying Proteins

Chromatin Biology
Research Category
Epigenetics & Nuclear Function

质量

Immunocytochemisty:
Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution).

目标描述

~180 kDa

外形

Protein G Chromatography
Format: Purified
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式purified antibody
antibody product typeprimary antibodies
克隆polyclonal
species reactivitymouse, human
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登记号NP_001073893
UniProt登记号O15054
运输wet ice
Gene Informationhuman ... KDM6B(23135)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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