产品说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
Broad species cross-reactivity is expected.
Recognizes dimethylated Histone H3 (Lys4), MW ~17 kDa. Specificity demonstrated by ELISA using methylated peptides.
免疫原
KLH conjugated synthetic peptide (ARTMe2KQTAR-GC) corresponding to amino acids 1-8 of human Histone H3.
Epitope: dimethylated Histone H3 (Lys4)
应用
Use Anti-dimethyl-Histone H3 (Lys4) Antibody (rabbit polyclonal antibody) is published and validated in ChIP, DB, ICC, IF, WB, PIA, ChIP-seq. to detect dimethyl-Histone H3 (Lys4) also known as H3K4me2, Histone H3 (di methyl K4).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Chromatin Immunoprecipitation:
5 μL of a previous lot immunoprecipitated methylated Histone H3 from 1 x 106 3T3/A31 cells.
Immunocytochemistry:
1:500 dilution of a previous lot showed positive immunostaining for methylated Histone H3 in L6 cells fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100.
质量
Evaluated by western blot on HeLa acid extract.
Western Blot Analysis: 1:2000-1:10,000 dilution of this antibody detected methylated Histone H3 in acid extracted proteins from HeLa cells.
1:2000-1:5000 dilution of a previous lot detected methylated Histone H3 in acid extracted proteins from HeLa and tetrahymena cells.
目标描述
Approx. 17 kDa
外形
Format: Unpurified
Rabbit polyclonal antiserum containing 0.05% sodium azide.
Unpurified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
HeLa acid extract
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | unpurified |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human, Tetrahymena sp. |
manufacturer/tradename | Upstate® |
technique(s) | ChIP: suitable (ChIP-seq) dot blot: suitable immunocytochemistry: suitable immunofluorescence: suitable inhibition assay: suitable (peptide) western blot: suitable |
NCBI登记号 | NP_003484.1 |
UniProt登记号 | Q16695 |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |