产品说明
一般描述
HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA binding protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK..
特异性
HuR
免疫原
KLH-conjugated, synthetic peptide corresponding to amino acids 1-13 of human HuR.
应用
Immunoprecipitation Analysis:
10 µg from a representative lot detected HuR in RIPA lysate.
Immunocytochemistry Analysis:
A 1:500 dilution from a representative lot detected HuR in HeLa and NIH/3T3 cell lysates.
Immunohistochemistry Analysis:
This antibody has been reported by an independent laboratory to detect HuR in rabbit placental tissue.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
RNA Binding Protein (RBP)
Anti-HuR Antibody is a Rabbit Polyclonal Antibody for detection of HuR also known as ELAV-like protein 1, Hu-antigen R & has been validated in IP, WB, ICC.
质量
Western Blot Analysis: Evaluated by Western Blot in 3T3/A31 cell lysate using 1 µg/mL of antibody and 10 µg of lysate.
目标描述
~36 kDa
外形
Format: Purified
See datasheet for specific purification method.
See datasheet for exact formulation.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析说明
Control
3T3/A31 cell lysate
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | mouse, human |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NP_001410 |
UniProt登记号 | Q15717 |
运输 | wet ice |
Gene Information | human ... ELAVL1(1994) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |