产品说明

一般描述

REDTaq^® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.

应用

For routine PCR amplifications REDTaq^® DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.

特点和优势

• 在实现与 Taq DNA 聚合酶相同性能的同时，具有适用于高通量应用的更方便形式。
• 目视确认不仅添加了酶，而且发生了适当的混合。
• 不需要额外的上样染料。可直接从反应中取一等份，上样到琼脂糖凝胶上进行电泳。

包装

提供含 MgCl _{2 的 10X 反应缓冲液}

单位定义

一个单位在 74℃ 下 可在30 分钟内将10 nmol 总 dNTP融入酸可沉淀 DNA 中。

法律信息

本产品的使用涵盖在以下一项或多项美国专利以及美国以外的相应专利要求中：美国 8,404,464 和美国7,972,828。本产品的采购包括上述专利要求下的有限、不可转让的诉讼豁免。
REDTaq is a registered trademark of sigma-aldrich Co. LLC

基本信息

产品性质

安全信息