产品说明
细胞系来源
Mouse C-1300 Neuroblastoma
细胞系描述
Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.
应用
Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus
培养基
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37℃.
其他说明
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产品性质
| 生物来源 | mouse nerve |
| 生长模式 | Adherent |
| 核型 | Unstable |
| 形态学 | Neuroblast |
| 产品 | Acetylcholinesterase, choline acetylase, tyrosine hydroxylase |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
