F9

产品说明

细胞系来源

Mouse 129 embryo teratocarcinoma

细胞系描述

Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.

应用

Differentiation studies

培养基

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂, 37℃. Upon resuscitation, cells shed large quantities of debris, leave for 2 days before subculture. Please note: Culture v

其他说明

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产品性质