产品说明
细胞系来源
A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line
DNA图谱分析
Not specified
培养基
RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS
传代培养常规
Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
产品性质
| 生物来源 | rat pancreas (islets) |
| 生长模式 | Adherent |
| 核型 | Modal chromosome number 66 up to passage 50 |
| 形态学 | Epitheloid |
| 产品 | BRIN-BG7 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG7 cells express the GLUT2 glucose transporter. |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
