产品说明
细胞系描述
Mouse embryonic fibroblasts (MEFs) derived from the ULK1(+/-) ULK2(+/-) knockout mice. MEFs were isolated and cultured and immortalised with SV40 large T-antigen. This cell line can be used to study ULK1 dependent processes in autophagy.
培养基
DMEM + 10% FCS +2mM Glutamine
传代培养常规
Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 104 cells per cm2. Cultures must be incubated in a humidified 5% CO2/95% air incubator at 37oC.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
免责声明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
产品性质
| 生物来源 | mouse embryo |
| 描述 | Mouse embryonic fibroblast, autophagy, Unc51-like kinase, SV40-immortalised |
| 生长模式 | Adherent |
| 形态学 | Fibroblast |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
