产品说明
一般描述
A cell-permeable inhibitor of caspase-3. The C-terminal DEVD-CHO sequence of this peptide is a highly specific, potent, and reversible inhibitor of caspase-3 (Ki<1 nM) that has also been shown to strongly inhibit PARP cleavage in cultured human osteosarcoma cell extracts (IC50 = 200 pM). The N-terminal sequence (residues 1-16) corresponds to the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF) and confers cell-permeability to the peptide.
包装
1 mg in Glass bottle
Packaged under inert gas
生化/生理作用
Primary Target
caspase-3
Target IC50: 200 pM inhibiting PARP cleavage in cultured human osteosarcoma cell extracts
Product does not compete with ATP.
Reversible: yes
Cell permeable: yes
警告
Toxicity: Irritant (B)
序列
Ac-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Asp-Glu-Val-Asp-CHO
外形
A 5 mM (1 mg/100 µl) solution of Caspase-3 Inhibitor I, Cell-permeable (Cat. No. 235423) in DMSO.
重悬
Following initial thaw, aliquot, purge with inert gas, and freeze (-20°C).
其他说明
Lin, Y.-Z., et al. 1996. J. Biol. Chem. 271, 5305.
Nicholson, D.W. 1996. Nature Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem.271, 1841.
Lin, Y.-Z., et al. 1995. J. Biol. Chem.270, 14255.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Lazebnik, Y.A., et al. 1994. Nature371, 346.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C94H158N20O27 |
分子量 | 2000.38 |
产品性质
质量水平 | 100 |
测定 | ≥90% (HPLC) |
形式 | liquid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze desiccated (hygroscopic) protect from light |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | 188.6 °F - (Dimethylsulfoxide) |
闪点(C) | 87 ℃ - (Dimethylsulfoxide) |