产品说明
一般描述
Selective and cell-permeable inhibitor of MAP kinase kinase (MEK) that acts by inhibiting the activation of MAP kinase and subsequent phosphorylation of MAP kinase substrates. Pretreatment of PC-12 cells with PD 98059 completely blocks the 4-fold increase of MAP kinase activity produced by nerve growth factor (NGF; IC50 = 2 µM); however, it has no effect on NGF-dependent tyrosine phosphorylation of the pp140trk receptor or its substrate Shc and does not block NGF-dependent activation of PI 3-kinase. Inhibits cell growth and reverses the phenotype of ras-transformed BALB 3T3 mouse fibroblasts and rat kidney cells.
包装
1 ml in Glass bottle
Packaged under inert gas
生化/生理作用
Cell permeable: yes
Target IC50: 2 µM in completely blocking the 4-fold increase in MAP kinase activity produced by nerve growth factor in PC12 cells
Primary Target
MAP kinase kinase (MEK)
Product does not compete with ATP.
Reversible: yes
警告
Toxicity: Irritant (B)
外形
A 5 mg/ml solution of PD 98059 (Cat. No. 513000) in anhydrous DMSO.
重悬
Following initial thaw, aliquot and freeze.
其他说明
Kültz, D., et al. 1998. J. Biol. Chem.273, 13645.
Dudley, D.T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 7686.
Langlois, W.J., et al. 1995. J. Biol. Chem.270, 25320.
Pang, L., et al. 1995. J. Biol. Chem.270, 13585.
Waters, S.B., et al. 1995. J. Biol. Chem.270, 20883.
Selected Citations
Lee, J., et al. 2009.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C16H13NO3 |
| 分子量 | 267.28 |
| MDL编号 | MFCD00671789 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥98% (HPLC) |
| 形式 | liquid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze protect from light |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| InChI | 1S/C16H13NO3/c1-19-14-8-4-6-11(16(14)17)15-9-12(18)10-5-2-3-7-13(10)20-15/h2-9H,17H2,1H3 |
| InChI key | QFWCYNPOPKQOKV-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
