产品说明
一般描述
A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.
包装
1 mg in Glass bottle
Packaged under inert gas
生化/生理作用
Cell permeable: yes
Primary Target
Monitor intracellular pH changes
Product does not compete with ATP.
Reversible: no
警告
Toxicity: Irritant (B)
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
其他说明
Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| MDL编号 | MFCD00036969 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥85% (mixture of isomers, HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze protect from light |
| 颜色 | white to off-white |
| 溶解性 | acetonitrile: 25 mg/mL DMF: soluble DMSO: soluble |
| 荧光 | λex 500-504 nm λem 525-531 nm |
| 运输 | ambient |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
