SiHa人子宫颈鳞癌细胞(2019年新引进)

产品介绍

目录号 Serial	SCSP-5058
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5058
描述 Description	电镜观察显示SiHa的细胞连接处具有典型的桥粒，胞浆中有丰富的张力丝。据报道，该细胞系整合一段人乳头瘤病毒16型基因组(HPV-16，每个细胞1到2个拷贝)。癌基因pRB和p53表达阳性。每个批次均通过本库支原体检测，结果阴性。经本库STR检测结果无误。STR结果如下：Amelogenin: X；CSF1PO: 12；D13S317: 11；D16S539: 12；D5S818: 9；D7S820: 10；THO1: 6,9；TPOX: 8；vWA: 14,17。
动物种别 Organism	人；亚洲人(日本人)
性别 Gender	女性，55岁
组织来源 Tissue and Cell Type	子宫颈
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	完全培养液配方(100 ml) MEM (Gibco, 11090081) 90 ml Glutamax (Invitrogen, 35050061) 1 ml Non-essential Amino Acids, 100× (Invitrogen, 11140050) 1 ml FBS (Gibco) 10 ml 参考传代周期：2-3天参考传代比例：1:3 参考换液频率：每周2次气相：空气，95%；二氧化碳，5%。温度：37摄氏度。冻存液配方：完全培养液95%，DMSO 5%
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430 Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Friedl F, et al. Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology. Proc. Soc. Exp. Biol. Med. 135: 543-545, 1970. PubMed: 5529598 Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 Olive PL, Banath JP. Multicell spheroid response to drugs predicted with the comet assay. Cancer Res. 57: 5528-5533, 1997. PubMed: 9407963

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