SK-N-SH人神经母细胞瘤细胞(2017年新引进)

产品介绍

目录号 Serial	SCSP-5029
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5029
描述 Description	人神经母细胞瘤细胞SK-N-SH由J.L.Bieder建系。与SK-N-MC所不同的是，SK-N-SH细胞倍增时间较长且多巴胺-β-羟基酶水平较高。SK-N-SH在细胞介导的细胞毒性试验中用作靶细胞系。我库冻存时为P42代。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：Amelogenin: X；CSF1PO: 11；D13S317: 11；D16S539: 8,13；D5S818: 12；D7S820: 7,10；THO1: 7,10；TPOX: 8,11；vWA: 14,18
动物种别 Organism	人
性别 Gender	女性，4岁
组织来源 Tissue and Cell Type	脑；来自转移灶：骨髓
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	人神经母细胞瘤细胞SK-N-SH完全培养液配方(100 ml)： MEM(invitrogen 11090081)87 ml FBS(Gibco)10 ml Gluta-max(invitrogen 35050061) 1 ml NEAA (Invitrogen, 11140050) 1 ml Sodium pyruvate(invitrogen 11360070) 1 ml 参考传代周期：3～5天一次参考传代比例：1:3-1:5 参考换液频率：每周2次冻存液配方：完全培养液95%，DMSO 5% 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
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