SK-NEP-1人肾母细胞瘤细胞
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目录号 Serial | TCHu164 | 标识符 Identifier | CSTR:19375.09.3101HUMTCHu164 | 描述 Description | 超微结构有少许微绒毛,连接复合物,形态完整的高尔基体,内质网多为光滑型,脂滴,没有病毒棵粒。 在本库通过支原体检测。 在本库通过STR检测。 | 动物种别 Organism | 人 | 性别 Gender | 女 | 组织来源 Tissue and Cell Type | 肾,肋膜渗出液 | 形态 Morphology | 圆形悬浮 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | McCOY's 5A(SIGMA,货号M4892,添加NaHCO3 2.2g/L),85%;优质胎牛血清,15%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press; 1975, pp. 115-159.Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047Orkin SH , et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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