MNNG/HOS Cl #5 [R-1059-D]人骨肉瘤细胞

产品介绍

目录号 Serial	TCHu167
标识符 Identifier	CSTR:19375.09.3101HUMTCHu167
描述 Description	这株细胞是HOS经0.01mcg/ml MNNG(一种致癌的亚硝胺)转化而来。这株细胞饱和浓度高，在软琼脂中的克隆形成率高，并能在裸鼠中成瘤。在本库通过支原体检测。在本库通过STR检测。
动物种别 Organism	人
性别 Gender	女
组织来源 Tissue and Cell Type	骨；骨肉瘤
形态 Morphology	***
培养基和添加剂 Complete Growth Medium and Culture Conditions	MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Rhim JS , et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046Rhim JS , et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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