MS751人子宫颈表皮癌细胞(2014年新引进)

产品介绍

目录号 Serial	SCSP-537
标识符 Identifier	CSTR:19375.09.3101HUMSCSP537
描述 Description	人子宫颈表皮癌细胞MS751由J. Sykes于1974年建立，来源于一位47岁白人女性。该细胞含有人乳头状瘤病毒18(HPV-18)序列。最近发现MS751细胞包含部分来源于E6/E7区域的HPV-45基因序列，表达poly(A)+RNA的形式。生物安全等级：二级。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 12；D13S317: 12；D7S820: 9,11；D16S539: 11；vWA: 16；THO1: 6；Amelogenin: X；TPOX: 8；CSF1PO: 11,12
动物种别 Organism	人；白人
性别 Gender	女性，47岁
组织来源 Tissue and Cell Type	子宫颈；分离自转移灶：淋巴结
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	Eagle MEM (Invitrogen, 11090081) 88 ml FBS (Gibco) 10 ml Glutamax(invitrogen 35050061) 1 ml Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 Geisbill J, et al. Detection and characterization of human papillomavirus type 45 DNA in the cervical carcinoma cell line MS751. J. Gen. Virol. 78: 655-658, 1997. PubMed: 9049418 Smith KJ, et al. The APC gene product in normal and tumor cells. Proc. Natl. Acad. Sci. USA 90: 2846-2850, 1993. PubMed: 8385345 Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at

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