MRC-5人胚肺细胞

产品介绍

目录号 Serial	GNHu41
标识符 Identifier	CSTR:19375.09.3101HUMGNHu41
描述 Description	MRC-5细胞系来自14周龄男性胎儿的正常肺组织，该细胞老化前能传代42到46次群体倍增。它是正常二倍体细胞系，46，XY核型。模式染色体数为46，概率为70%。在本库通过支原体检测。在本库通过STR检测。
动物种别 Organism	人
性别 Gender	男
组织来源 Tissue and Cell Type	肺
形态 Morphology	成纤维细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Jacobs JP , et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970, pp. 43-55.Holliday R , Tarrant GM . Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262Lewis CM , Tarrant GM . Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029Kaur A , et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893MOLECULAR THERAPY 11(4):531,2005

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