HT-29人结肠癌细胞
产品介绍
目录号 Serial | TCHu103 | 标识符 Identifier | CSTR:19375.09.3101HUMTCHu103 | 描述 Description | 1964年,HT-29细胞系由J.Fogh 用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离。 近来,已建株的培养细胞用含相同血清McCoy’s 5a培液培养。 该细胞系在裸鼠中致瘤,也能在类固醇处理的地鼠中致瘤。 这个细胞株合成IgA, CEA, 蛋白绑定TGFβ的分泌成分和粘液素。 在本库通过支原体检测。 在本库通过STR检测。 | 动物种别 Organism | 人 | 性别 Gender | 女 | 组织来源 Tissue and Cell Type | 结直肠腺癌 | 形态 Morphology | 上皮细胞 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | McCOY's 5A(SIGMA,货号M4892,添加NaHCO3 2.2g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Journal of Interferon & Cytokine Research 24:219,2004.Didier ES , et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708editors. Human tumor cells in vitro. New York: Plenum Press; 1975, pp. 115-159.Chen TR , et al. 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Differential expression of the human mucin genes MUC1 to MUC5 in relation to growth and differentiation of different mucus-secreting HT- 29 cell subpopulations. J. Cell Sci. 106: 771-778, 1993. PubMed: 8308060Pollack MS , et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212Fantini J , et al. Infection of colonic epithelial cell lines by type 1 human immunodeficiency virus is associated with cell surface expression of galactosylceramide, a potential alternative gp120 receptor. Proc. Natl. Acad. Sci. USA 90: 2700-2704, 1993. PubMed: 8464878Devedjian JC , et al. Regulation of the alpha 2A-adrenergic receptor in the HT29 cell line. Effects of insulin and growth factors. J. Biol. Chem. 266: 14359-14366, 1991. PubMed: 1677644Santoro IM , Groden J . Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. 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