HuH-7人肝癌细胞(肝脏)

产品介绍

目录号 Serial	SCSP-526
标识符 Identifier	CSTR:19375.09.3101HUMSCSP526
描述 Description	有报道称人肝癌细胞HuH-7可以无血清培养，会在培养基内分泌许多血清蛋白和alpha phyto蛋白。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 12；D13S317: 10,11；D7S820: 11；D16S539: 10；vWA: 16,18；THO1: 7；Amelogenin: X；TPOX: 8,11；CSF1PO: 11
动物种别 Organism	人
性别 Gender
组织来源 Tissue and Cell Type	肝脏
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	DMEM (Invitrogen, 11960-044) 88ml FBS (Gibco) 10ml Glutamax (Invitrogen, 35050-061) 1ml Sodium Pyruvate 100 mM Solution (invitrogen 11360070) 1ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs.Randall G,Grakoui A,Rice CM Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):235-40Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication.Blight KJ,McKeating JA,Rice CMJ Virol. 2002 Dec;76(24):13001-14 Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.Lohmann V,K�rner F,Koch J,Herian U,Theilmann L,Bartenschlager R Science. 1999 Jul 2;285(5424):110-3Establishment and characteristics of human hepatocellular carcinoma cells with metastasis to lymph nodes.Seki S,Kitada T,Kawada N,Sakaguchi H,Kadoya H,Nakatani K,Satake K,Kuroki T Hepatogastroenterology. 1999 Sep-Oct;46(29):2812-7Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium.Nakabayashi H,Taketa K,Yamane T,Oda M,Sato J Cancer Res. 1985 Dec;45(12 Pt 1):6379-83Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.Nakabayashi H,Taketa K,Yamane T,Miyazaki M,Miyano K,Sato J Gann. 1984 Feb;75(2):151-8Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J Cancer Res. 1982 Sep;42(9):3858-63

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