Jurkat, Clone E6-1人白血病T淋巴细胞(2018年新引进)

产品介绍

目录号 Serial	SCSP-513
标识符 Identifier	CSTR:19375.09.3101HUMSCSP513
描述 Description	人白血病T淋巴细胞，Jurkat细胞由Schneider建立，来源于一个14岁的男孩的外周血。该克隆是 Jurkat-FHCRC细胞株的一个克隆(Jurkat的一个衍生株)，经佛波酯(phorbol esters)和外源凝集素(lectins)或抗T3单克隆抗体(需要两种物质共同诱导)诱导后可产生大量IL-2。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 9；D13S317: 8,12；D7S820: 8,12；D16S539: 11；vWA: 18；THO1: 6,9.3；Amelogenin: X,Y；TPOX: 8,10；CSF1PO: 11,12
动物种别 Organism	人
性别 Gender	男性
组织来源 Tissue and Cell Type	外周血
形态 Morphology	成淋巴细胞状，悬浮生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	1. 人白血病T淋巴细胞Jurkat, Clone E6-1完全培养液配方(100 ml) RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml FBS (Gibco) 10 ml Glutamax(invitrogen 35050-061) 1 ml Sodium Pyruvate 100 mM Solution (invitrogen 11360070) 1 ml 参考传代周期：不超过300万细胞 /mL，建议将活细胞密度控制在10万-100万/mL之间参考传代密度：10万活细胞/mL 参考换液频率：2-3天冻存液：完全培养液95%，DMSO 5% 细胞状态：悬浮生长气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 2. 注意事项： a) 该细胞为悬浮细胞，根据培养经验以及客户的反馈，传代时使用【半换液法】对细胞状态较为有利，因此我库建议您使用【半换液法】进行传代。同时，您在收到细胞后，请不要通过离心的方式收集细胞，可以直接向培养瓶中添加等体积的新鲜培养液，然后将细胞吹打均匀后移入两个新的T25培养瓶中继续培养即可。 b) 细胞对血清质量较为敏感，我库建议您使用进口大品牌优质血清进行培养。 c) 该细胞对细胞密度较为敏感，培养、传代时请注意保持细胞密度在合适的范围。
供应限制 Permits and Restrictions	A。仅供研究之用。
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