K-562人慢性髓系白血病细胞(2019年新引进)

产品介绍

目录号 Serial	SCSP-5054
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5054
描述 Description	连续细胞株K-562来源于一位临终的53岁女性慢性髓性白血病患者的胸水。细胞被归入高度未分化的粒性白细胞类。Anderson等对其表面膜性质的研究表明，K-562是一株人类红白血病细胞。K-562母细胞是多能性，造血恶性细胞，它能自发分化成可识别的红血球祖细胞、粒性粒细胞、单核细胞等。Dimery等将多种K-562亚系分为3类(A,B,C)，此株细胞最接近B类，出现费城染色体，但频次较低，在15个分裂中期检测中未检测到。它是EBNA阴性的。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X；CSF1PO: 9,10；D13S317: 8；D16S539: 11,12；D5S818: 11,12；D7S820: 9,11；THO1: 9.3；TPOX: 8,9；vWA: 16。
动物种别 Organism	人
性别 Gender	女性，53岁
组织来源 Tissue and Cell Type	骨髓
形态 Morphology	成淋巴细胞样，悬浮生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	1.人慢性髓系白血病细胞K-562完全培养液配方(100 ml)： IMDM (Invitrogen, 31980-030)90 ml FBS (Gibco)10 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。参考传代比例：细胞接种密度10万个/mL，维持密度10万-100万个/mL. 参考换液频率：每周2次冻存液配方：完全培养液95%，DMSO 5% 或冻存液：厂商：ZENOAQ，货号：CellBanker2。 2. 注意事项： a) 该细胞为悬浮细胞，根据培养经验以及客户的反馈，传代时使用【半换液法】对细胞状态较为有利，因此我库建议您使用【半换液法】进行传代。同时，您在收到细胞后，请不要通过离心的方式收集细胞，可以直接向培养瓶中添加等体积的新鲜培养液，然后将细胞吹打均匀后移入两个新的T25培养瓶中继续培养即可。 b) 细胞对血清质量较为敏感，我库建议您使用进口大品牌优质血清进行培养。 c) 该细胞对细胞密度较为敏感，培养、传代时请注意保持细胞密度在合适的范围。细胞接种密度10万个/mL，维持密度10万-100万个/mL.
供应限制 Permits and Restrictions	A。仅供研究之用。
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