HT-29人结肠癌细胞(2017年新引进)

产品介绍

目录号 Serial	SCSP-5032
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5032
描述 Description	HT-29细胞表达尿激酶受体，但未检测到纤溶酶原激活物活性[PubMed ID:8381394]。HT-29 细胞CD4表达阴性，但细胞表面表达半乳糖神经酰胺(一个HIV的可替代受体)。致癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos表达阳性，过量产生p53抗原，在p53基因的273密码子处有G->A突变导致Arg->His替换，未检测到致癌基因N-myc的表达。本库冻存时为P140左右。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 11,12；D13S317: 11,12；D7S820: 10；D16S539: 11,12；vWA: 17,19；THO1: 6,9；Amelogenin: X；TPOX: 8,9；CSF1PO: 11,12
动物种别 Organism	人
性别 Gender	女性，44岁
组织来源 Tissue and Cell Type	结肠
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	1. 人结肠癌细胞细胞HT-29完全培养液配方(100 ml)： McCoy's 5a Medium (Invitrogen, 12330-031) 90 ml FBS (Gibco) 10 ml 气相：空气95%，二氧化碳5%；37℃培养。传代比例：1：3 传代周期：3-4天换液频率：每周2次冻存液：完全培养液95%，DMSO 5% 细胞状态：贴壁生长 2. 注意事项： HT-29复苏后**周可能会有大量漂浮细胞，可更换新鲜培养液，同时将漂浮的细胞离心收集后加回到培养瓶中与贴壁细胞共同培养。新复苏的细胞会以补丁形态三维接触生长(即不是单层扁平生长)，待细胞分裂生长后将会变回上皮细胞形态单层扁平生长。
供应限制 Permits and Restrictions	A。仅供研究之用。
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