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Caco-2 [Caco2]人结直肠腺癌细胞
产品介绍
目录号
Serial
TCHu 146
标识符
Identifier
CSTR:19375.09.3101HUMTCHu146
描述
Description
这株细胞分离自直肠原位癌。 当长到满时,细胞表现出特征性的肠上皮细胞分化。 Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II,并呈角质蛋白阳性。
在本库通过支原体检测。
在本库通过STR检测。
动物种别
Organism
性别
Gender
组织来源
Tissue and Cell Type
结肠,结直肠腺癌
形态
Morphology
上皮细胞
培养基和添加剂
Complete Growth Medium and Culture Conditions
MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),80%;优质胎牛血清,20%。
气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCEDidier, E.S., et al. Characterization of Encephalitozoon (Septata)  intestinailis  isolates  cultured  from  nasal mucosa and bronchoalveolar lavage fluids of two AIDSpatients. J. Eukaryot. Microbiol 43: 34-43. 1996 PubMed:96151459Jumade, C., and Malo, C. Caco-2 cells cultured in serum-free  medium  as  a  model  for  the  study  of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991 PubMed: 92042315Fogh, J., et al. Absence of HeLa ceil contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977 PubMed: 77097006Fogh,  J.,  et al.  One  hundred  and  twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977 PubMed:77210034Adachi, A., et al. Productive, persistent infection of human    co]orectal    cell     lines    with    human immunodeficiency virus.  J.  Virol.  61:  209-213,  1987 PubMed: 87061242Trainer, D.L., et al. Biological characterization and oncogene expression  in  human colorectal  carcinoma cell  lines.  Int.  J.  Cancer 41:  287-296.  1988  PubMed:88114170Cohen, M.B., et al. Receptors for Escherichia coil heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell, Physiol. 156: 138-144,1993 PubMed: 93390921Basson, M.D., et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994 PubMed: 94357990Heinen,  C.D.,  et  al.  Microsatellite  instability  in colorectal adenocareinoma cell lines that have full-length adenomatous polyposis coil protein. Cancer Res. 55: 4797-4799, 1995 PubMed: 96046575GilberL, T., Rodriguez-Boulan, E. Induction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line. J. Cell Sci. 100: 451-458, 1991 PubMed: 92226167Rigothier,  M.C.,  et  al.  A  new  in  vitro  model  of Entamoeba histolytica adhesion, using the human colon carcinoma   cell   line   Caco-2:   scanning   electron microscopic study. Infect. Immun. 59: 4142-4146, 1991 PubMed: 92040089Levin,  M.S.  Cellular  retinol-binding  proteins  are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. J. Biol. Chem. 268: 8267-8276.1993 PubMed: 93216811Trotter, P.J., and Storch, J. Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023, 1993 PubMed: 93252877Santoro, I.M., Groden, J. Alternative splicing of the APC   gene   and   its   association   with   terminal differentiation. Cancer Res. 57: 488-494, 1997 PubMed:97164672White, L.J., et al. Attachment and entry of recombinant norwalk virus eapsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996 PubMed: 96386543Baier,  L.J.,  et al. A polymorphism in the  human intestinal fatty acid binding protein alters fatty acid transport across Caco-2 cells. J. Biol. Chem. 271: 10892-19896. 1996 PubMed: 96210027Kutchera, W., et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93:4816-4820, 1996 PubMed: 96209815Grindstaff, K.K. et al. Translational regulation of Na,K-ATPase alpha1  and beta1  polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996 PubMed: 96394411Hay, R. J., Caputo, J. L., and Macy, M. L. Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L.,  Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers  for  Disease  Control  (1993),  Biosafety  in Microbiological  and  Biomedical  Laboratories  Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
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