Caco-2 [Caco2]人结直肠腺癌细胞(2017年新引进)

产品介绍

目录号 Serial	SCSP-5027
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5027
描述 Description	人结直肠腺癌细胞Caco-2 [Caco2]分离自直肠原位癌，适于转染。当细胞长满时，可表现出肠上皮细胞分化的特征。Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II。每个批次均通过本库支原体检测，结果为阴性。冻存时为P30代。经本库STR检测无误，STR鉴定结果为：Amelogenin: X,CSF1PO: 11,D13S317: 11,13,14,D16S539: 12,13,D5S818: 12,13,D7S820: 11,12,THO1: 6,TPOX: 9,11,vWA: 16,18
动物种别 Organism	人；白人
性别 Gender	男性，72岁
组织来源 Tissue and Cell Type	结肠
形态 Morphology	贴壁生长；细胞形态不均一，有部分含有液泡的巨大细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	人结直肠腺癌细胞Caco-2完全培养液配方(100 ml)： MEM (Invitrogen，11090081) 77 ml FBS (Gibco) 20 ml Glutamax(invitrogen 35050061) 1 ml Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml 参考传代周期：4-6天参考传代比例：1 : 3 冻存液：完全培养液95%，DMSO 5% 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。注意事项： 1.复苏或传代后细胞贴壁较慢，大量细胞会漂浮并存在一些碎片，最终会以岛状形态贴壁并扩散生长。为了避免干扰细胞贴壁，48h内不要换液；细胞形态不均一，有一些含有液泡的巨大细胞，该细胞培养过程中会有黑色颗粒物，属正常现象；消化时间5-10 min。 2.该细胞对血清质量较为敏感，我库建议您使用品牌优质胎牛血清进行培养或选择订购我库配套Caco-2完全培养液，货号为SCSP-671。 3.我库冻存时，每支冻存管约含1×106细胞量，体积为500μl，预期存活率70%，建议复苏至1个T25培养瓶中。
供应限制 Permits and Restrictions	A。仅供研究之用。
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