Caki-1人肾透明细胞癌细胞(2019年新引进)
产品介绍
目录号 Serial | SCSP-5064 | 标识符 Identifier | CSTR:19375.09.3101HUMSCSP5064 | 描述 Description | 超微结构的特征包括一些微绒毛、少许细丝、一些小线粒体、发达的高尔基体和内质网、一些脂滴、多层体和次级溶酶体,无病毒颗粒。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测无误,STR鉴定结果为:Amelogenin: X; CSF1PO: 10,11; D13S317: 11,12; D16S539: 12; D5S818: 11,12; D7S820: 8,12; THO1: 6,8; TPOX: 8,11; vWA: 15,17 | 动物种别 Organism | 人;白人 | 性别 Gender | 男性,49岁 | 组织来源 Tissue and Cell Type | 肾;来自转移灶:皮肤 | 形态 Morphology | 上皮样,贴壁生长 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | 人肾透明细胞癌细胞Caki-1完全培养液配方(100 ml): McCoy's 5A(Gibco, 16600082) 90 ml FBS (Gibco) 10 ml 参考传代周期:每周2-3次 参考传代比例:1:2-1:4 参考换液频率:每周2-3次 冻存液配方:完全培养液95%,DMSO 5% 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |
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