产品说明
一般描述
Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.
应用
Sulfatase from Aerobacter aerogenes has been used:
- as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
- in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
- to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides
生化/生理作用
Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.
单位定义
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 ℃.
外形
Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
基本信息
| 酶学委员会(EC)编号 | 3.1.6.1 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00132399 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 类型 | Type VI |
| 形式 | buffered aqueous glycerol solution |
| specific activity | 2-5 units/mg protein (biuret) |
| 分子量 | ~41 kDa |
| 浓度 | 10-20 units/mL |
| 异质活性 | β-Glucuronidase ≤10 U/mL |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
