产品说明
一般描述
N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.
应用
N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.
包装
1 kit containing 3 components
制备说明
Working solution: Preparation of solutions:
- Buffer solution
Dissolve the contents of bottle 1 with 55 ml double-distilledwater. - Substrate solution
Dissolve the contents of bottle 2 with 55 ml solution I. - Stop reagent
Dissolve the contents of bottle 3 with 110 ml double-distilled water.
Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 ℃, protected from light.
Solution III is stable for 1 month stored at 2 to 8 ℃.
Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 ℃ and for one month when stored at -15 to -25 ℃.
其他说明
For life science research only. Not for use in diagnostic procedures.
产品性质
| 用途 | sufficient for ~50 tests |
| 质量水平 | 100 |
| manufacturer/tradename | Roche |
| 运输 | ambient |
| 储存温度 | room temp |
组份列表
组份不可单独销售
| 货号 | 组份 |
| Buffer Substance consisting of citric acid and potassium citrate | |
| Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax | |
| Stop Reagent consisting of sodium carbonate |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H319 - H360FD |
| 预防措施声明 | P201 - P202 - P280 - P308 + P313 - P337 + P313 - P501 |
| 危险分类 | Eye Irrit. 2 - Repr. 1B |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 2 |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |
