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Anti-P2X2 Receptor Antibody

品牌
Sigma-Aldrich
货号
AB5894
规格纯度
serum, Chemicon®
参考价格
5184.65 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

P2X2 Receptor.

免疫原

A 13 amino acid peptide corresponding to amino acids 460-472 from the carboxy-terminus of the rat P2X2 Receptor protein.

Control peptide: Catalog Number AG354

应用

Anti-P2X2 Receptor Antibody is an antibody against P2X2 Receptor for use in IC, IH & WB.
Immunoblotting: 1:500

Immunohistochemistry: 1:500.

Immunocytochemistry: 1:500.

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR AB5894

IMMUNOHISTOCHEMISTRY

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5894 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

IMMUNOCYTOCHEMISTRYP

2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above

WESTERN BLOTTING

Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8°C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with AB5894 diluted 1:500 with same buffer overnight at 2-8°C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

外形

Serum. Liquid. Contains 0.05% sodium azide.

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源guinea pig
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman, rat
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_012226.3
NM_016318.2
NM_170682.2
NM_170683.2
NM_174872.1
NM_174873.1
UniProt登记号Q9UBL9
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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