产品说明
一般描述
Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including; the identification and appropriation of splice-sites, and the manipulation of alternative splicing regulation.
免疫原
Purified oocyte nuclei containing xenopus SR proteins.
应用
Use Anti-Phosphoepitope SR proteins Antibody, clone 1H4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect Phosphoepitope SR proteins also known as Ser-Arg-rich proteins.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC (Fukuhara, T., et al. (2006).
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Buratti, E., et al. (2007).
Research Sub Category
RNA Metabolism & Binding Proteins
质量
Evaluated by Western Blot in L6 plus insulin cell lysate.
Western Blot Analysis: 0.025 µg/mL of this antibody detected SR proteins in 10 µg of L6 plus insulin cell lysate.
目标描述
~ 75, 55, 40, 30, and 20 kDa observed.
This antibody recognizes the family of SR proteins which consist of 75, 55, 40, 30, 20 kDa proteins.
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
L6 plus insulin cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 1H4, monoclonal |
species reactivity | rat |
species reactivity (predicted by homology) | mouse (based on 100% sequence homology), human (based on 100% sequence homology) |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1κ |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |