产品说明
一般描述
Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme possessing both endo- and exoglycosidase activity against poly (ADP-ribose) (PARP), rapidly degrading PARP to release large quantities of free ADP-ribose. PARG is involved in several cellular processes including; apoptosis, DNA repair, cell cycle progression, cell survival and cellular differentiation. During apoptosis, PARG is cleaved by caspase-3 suggesting that PARG activity is regulated during this process. Although encoded by one gene, PARG is present in different cellular localizations as different isoforms: Isoform 1 (111 kDa) is present in the nucleus, Isoform 2 (102 kDA) is in the cytoplasm and Isoform 3 (99 kDa) is mitochondrial. Studies have indicated a critical role for PARG isoform 1 in the quality of sperm chromatin, and subsequent embryonic survival.
免疫原
Histidine-tagged recombinant protein corresponding to human PARG.
Epitope: Unknown
应用
Anti-PARG Antibody, clone D8B10 is an antibody against PARG for use in WB & IP.
Research Category
Signaling
Epigenetics & Nuclear Function
Research Sub Category
Hormones & Receptors
Chromatin Biology
质量
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PARG on 10 µg of Jurkat cell lysate.
目标描述
~ 102 kDa observed. Other isoforms may be observed in some lysates at 111 and 99 kDa
外形
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Jurkat cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | D8B10, monoclonal |
| species reactivity | human |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG2aκ |
| NCBI登记号 | NP_003622 |
| UniProt登记号 | Q86W56 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
