Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2

产品说明

一般描述

p62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis.

免疫原

GST-tagged recombinant protein corresponding to human p62 (Sequestosome-1).

应用

Research Sub Category
Apoptosis - Additional
Detect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC.
Western Blot Analysis: 0.001 µg/mL from a previous lot detected p62 (Sequestosome-1) in 10 µg of PC12 cell lysate.

Flow Cytometry Analysis: 1.0 µg from a previous lot detected p62 (Sequestosome-1) in the staining of fixed and permeabilized HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p62 (Sequestosome-1) in NIH/3T3, A431, and HeLa cells.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.001 µg/mL of this antibody detected p62 (Sequestosome-1) in 10 µg of A431 cell lysate.

目标描述

~47-60 kDa observed.
The calculated molecular weight of this protein is 47 kDa and also has an isoform at 38 kDa Due to modifications, this protein may be observed up to ~60 kDa in some lysates.

外形

Ion Exchange
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
A431 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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