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Anti-NeuroD 2 Antibody

品牌
Sigma-Aldrich
货号
AB5688
规格纯度
Chemicon®, from rabbit
参考价格
6861.94 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Neurogenic differentiation 2, also known as NEUROD2, is a human gene. This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates. Diseases associated with NEUROD2 are motor deficits, ataxia, and seizures. NEUROD2 expression is observed mainly in endovascular invasive cells.

特异性

Recognizes NeuroD 2 (NRDL, KW80), a Helix-loop-helix class of transcription factor.

免疫原

Synthetic peptide, amino acids 23-39 of human NeuroD2.

应用

Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:500 on PC12 cells
Western blot: 1:200-1:1,000 using ECL.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Anti-NeuroD 2 Antibody detects level of NeuroD 2 & has been published & validated for use in IC, IH, IH(P) & WB.
Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

目标描述

41 kDa

外形

Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

储存及稳定性

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The antibody must be "rehydrated"prior to use:
To rehydrate/reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium
sulfate/PBS solution: 10 mL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 mg of precipitated antibody, the amount of buffer needed would be 50 mL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody can be stored at:
2-8°C The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°.

-20°C For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

-70°C For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.

分析说明

Control
Cerebellum tissue

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman, rat, mouse
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI登记号NM_006160.3
UniProt登记号Q15784
运输dry ice
Gene Informationhuman ... NEUROD2(4761)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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