产品说明
一般描述
Histone MacroH2A (mH2A), also known as H2AFY, is a vertebrate-specific histone variant associated with condensed, inactive heterochromatin. This histone variant consists of a histone H2A-like domain fused to a large non-histone region. mH2A has been shown to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. The non-histone region of mH2A is directly involved in the repression of transcription, histone acetylation and blocking SWI/SNF and ACF nucleosome remodeling. This variant histone is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci.
免疫原
GST-tagged recombinant protein corresponding to human macro-H2A1.2.
应用
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)
Research Sub Category
Histones
Anti-macro-H2A1.2 Antibody, clone 14G7 is a Mouse Monoclonal Antibody for detection of macro-H2A1.2 also known as H2A histone family member Y or Histone macroH2A1 & has been validated in WB, ICC & IHC.
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected macro-H2A1.2 on 10 µg of MCF-7 cell lysate.
目标描述
~39 kDa observed
外形
Format: Purified
Purified mouse monoclonal IgG2bλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
MCF-7 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 14G7, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NP_001035248 |
UniProt登记号 | O75367 |
运输 | wet ice |
Gene Information | human ... H2AFJ(55766) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |