产品说明
特异性
IP3 Receptor 3.
免疫原
Synthetic peptide from the C-terminus of rat IP3 Receptor 3.
应用
Research Category
Signaling
Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 3 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Lipid Signaling
Anti-IP3 Receptor 3 Antibody detects level of IP3 Receptor 3 & has been published & validated for use in IC, IH & WB.
外形
Rabbit serum. Liquid.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
分析说明
Control
Western blot = adult mouse pancreas.
IHC = adult mouse hepatocytes (adult mouse cerebellum granule cells will be negative).
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | serum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | rat, human, mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
| NCBI登记号 | NM_002224.2 |
| UniProt登记号 | Q14573 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
